Assisted Hatching
IVF Treatments with latest Assisted Reproductive Technology is evolving every day. The area of human assisted reproductive technology advances at an astonishing rate. Assisted Reproductive Technology (ART) includes in vitro fertilization-embryo transfer (IVF-ET), gamete intrafallopian transfer (GIFT), zygote intrafallopian transfer (ZIFT), and frozen embryo transfer (FET). These techniques also apply to oocyte donation and gestational carriers.
In vitro fertilization (IVF) and other assisted reproductive technologies (ART) have become accepted medical treatments for infertility. Through these procedures, many couples with otherwise untreatable infertility have given birth to healthy babies.
Saturn 5 Active™ Laser System
Over recent years lasers have been used increasingly in the in vitro fertilization (IVF) laboratory. The procedures for which lasers are now routinely used include laser assisted hatching or thinning, blastomere biopsy, trophectoderm biopsy and polar body biopsy.
The latest addition to the Assisted Reproductive Technology is the Saturn 5™ Active Laser System. Saturn 5™ Active Laser System is the latest cutting-edge ART technology and is the most advanced, safest and fastest laser system for laser assisted hatching in repeated IVF failures and laser assisted biopsies of embryos for pre-implantation genetic diagnosis in recurrent missed abortions,IVF failures and to detect genetic abnormalities like Thalassemia, Down’s syndrome, Muscular Dystrophy, cystic fibrosis etc in infertile couples’ embryos.
By using Saturn 5™ or Saturn 5 Active™ laser systems, (the highest power lasers currently available), the embryologist can improve the safety of laser procedures compared with lower power systems. The Saturn 5 Active™ offers major new features for easier, safer and faster laser drilling for IVF procedures, benefiting all embryologists and embryos and our IVF Treatment patients.
The Saturn 5 Active™ directional laser system now comes with the new Biopsy Mode allowing accurate and automated laser drilling along a pre-defined line and Multi-Pulse option making procedures quick, accurate and effortless.
The new laser system makes the procedure quicker, easier and safer to perform than ever before.
The Saturn 5™ laser has really improved and sped up ART procedures.
We are pleased to introduce one of the latest facilities “LASER ASSISTED HATCHING” at Blossom IVF CENTRE.
Who should be treated with assisted hatching?
The most commonly used indications for assisted hatching with an in vitro fertilization case are:
Age factor – the female partner is older than 37
Egg quantity and quality factor – cases in which the woman’s day 3 follicle stimulating hormone (FSH) level is elevated,or with a low AMH level or low antral follicle count
Embryo quality factor – cases with poor quality embryos (excessive fragmentation or slow rates of cell division)
Zona factor – cases with embryos that have a thick outer shell (zona pellucida)
Previous IVF failure – cases that have had one or more previous failed IVF cycles.
Its time to take advantage of latest tachnology and Feel free to visit our centre between 10 AM to 6 PM to learn more about this technology and consult our fertility specialists Dr Praful Doshi,Dr Mitsu Doshi or Dr Rupal Shah.
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One of the most frustrating aspects of assisted reproductive technology for patients and fertility professionals alike is having to deal with failure in fertility treatment. This is especially true in couples who have attempted assisted reproductive procedures many times, and also in those whose time is running out because of their age. Now, a recently developed technique, assisted hatching, is offering new hope to the couples who fall into these categories.
Assisted hatching is a laboratory technique used with IVF. It involves the use of laser to thin the outer shell (zone pellucida) of the fertilized egg, before the embryo is transferred into the uterus. Assisted hatching is used to enhance the embryos ability to hatch, and also implant, after transfer. The procedure is based on the fact that an alteration in zona pellucida (outer covering of egg) either by drilling a hole through it or by thinning it, will promote hatching or implantation of embryos that are otherwise unable to escape intact from the zona pellucida.
After a fertilized egg is returned to the uterus, several things must happen:
- It must continue to divide and grow (it is now called an embryo).
- The embryo must break out of the zona pellucida (usually called the zona) which is a hard protein shell that surrounds it.
- The embryo must then burrow into the lining of the uterus (called the endometrium) and continue to grow there.
It has been suggested that making a hole in or thinning this outer layer may help embryos to ‘hatch’, increasing the chances of the woman becoming pregnant in some cases. Assisted hatching is indicated for infertile couples in following cases.
- when the zona pellucida is noted to be excessively thick when measured on a day 3 embryo by the embryologist
- with poor embryo quality
- In women with an elevated Day 3 FSH
- when a Flare stimulation protocol has been used
- when the female partner age is 38 or older at the time of stimulation, or
- with previous IVF implantation failure of embryos to implant though results were otherwise good
- Frozen embryo replacements.
- Laser Assisted Embryo Hatching
How does the procedure of Assisted Hatching work?
Assisted hatching is carried out in the laboratory by experienced embryologists. The assisted hatching procedure, like ICSI, is carried out by a technique known as micromanipulation. In small dishes the embryos, which now contain an average of six to eight cells, are stabilized by a holding pipette, while on the opposite side a small pipette containing acidified Tyrode’s solution creates a small defect in the zona. Just before the embryos are replaced, whether they are fresh or frozen/thawed, a small hole is made in the zona, or by thinning it using a micromanipulation technique. The process is repeated for each embryo. The main methods currently in use for assisted hatching are: chemical, mechanical and laser. The process will damage about 1% of embryos.
Most IVF clinics will not perform assisted hatching if there is one embryo available because of possible damage to this embryo would result in no embryo transfer. The assisted hatched embryos are then transferred into the uterine cavity as usual. Some clinics will give the woman a course of antibiotics to prevent infection. (Assisted hatching deprives the embryo of its intact protective coat, which shields it from exposure to any harmful factors in the uterus).
Assisted Hatching using laser technology is probably the best technique. Laser assisted hatching is a gentle and safe way to weaken a part of zona pellucida. Several studies have shown that using a laser is superior to chemical and manual hatching. Minimal handling of the embryo and delivering fast and exact control over the drilling of the hole are the advantages of laser assisted hatching.
Advantages of using Assisted Hatching
This relatively small variation in the IVF procedure has yielded dramatic results in older patients and those with previously failed cycles.
1) It provides “mechanical advantage” to the embryos in breaking the zona for implantation.
2) It also ensures early contact of embryos with endometrium. This expedites enzymetic reaction between endometrium and embryos called “Embryo endometrium cross talk”.
3) Mechanical advantages and chemical advantage ensure better implantation rate and better pregnancy rate.
4) It helps women with previous failed IVF cycle.
Disadvantages
The addition of assisted hatching to the standard IVF protocol does add extra laboratory manipulation and therefore added costs. There is a small risk of damage to the embryo during the micromanipulation process or at the time of transfer, and there is a slight increase in identical twinning. There is a greater chance of fetal complications and abnormalities in some identical twins. A rare complication of identical twinning is conjoined or “Siamese” twins. No higher rate of identical twins is observed than with routine IVF. This may relate to whether a large enough opening is made in the zona to prevent pinching of the embryo during the hatching process.
Assisted hatching of IVF embryos, is an Assisted Reproductive Technology procedure which is performed in IVF laboratory. It increases IVF success rates. Consult infertility expert at Blossom Fertility centre in Surat for complete medical advice for infertile couples.
At the Blossom Fertility and IVF Centre Surat, Laser Assisted Hatching technique is used to perform the assisted hatching procedure.Doctors and embryologist are well conversant with the latest technology in the field of ART (Assisted Reproductive Technology). You can contact the top IVF doctors, Fertility specialist Surat and embryologist in surat at Blossomivfindia.com or at IVFfertility-treatments
